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Nuclear extracts from Drosophila SL2 cells were found to contain a hypoxically inducible complex capable of binding to hypoxia response elements from mammalian genes. This complex (HIF-D) resembled mammalian hypoxia inducible factor (HIF-1) in DNA sequence specificity, abrogation of induction by cycloheximide, induction by desferrioxamine and redox sensitivity of DNA binding. However, HIF-D was not induced by cobalt and was less sensitive to phosphatase than HIF-1. Endogenous phosphoglycerate kinase mRNA in SL2 cells showed similar inducible characteristics to HIF-D. These findings are evidence that the mammalian HIF-1 dependent system of oxygen regulated gene expression has a functional homologue in Drosophila.

Type

Journal article

Journal

FEBS Lett

Publication Date

03/06/1996

Volume

387

Pages

161 - 166

Keywords

Animals, Base Sequence, Binding Sites, Cell Hypoxia, Cell Line, Cobalt, Cycloheximide, DNA Probes, DNA-Binding Proteins, Deferoxamine, Dithiothreitol, Drosophila melanogaster, HeLa Cells, Humans, Hypoxia-Inducible Factor 1, Hypoxia-Inducible Factor 1, alpha Subunit, Mice, Molecular Sequence Data, Nuclear Proteins, Phosphoglycerate Kinase, Phosphoric Monoester Hydrolases, Transcription Factors